Efficient degradation of various recalcitrant azo dyes in aqueous medium by immobilized Origanum vulgare peroxidase

Table 1 The efficacy of POX_ov immobilization on activated MHyX

Solution	V (mL)	Abs. 280 nm	Abs. 403 nm	Rate dA min⁻¹	Unit of Activity^b µM min⁻¹	Total Units of Activity µM min⁻¹
POX_ov (B.I.)	2	0.0813	0.089	0.108	7.05	7050
POX_ov (A.I.)	2	0.318	0.012	0.014	0.91	910
Wash	6	0.059	0.004	0.026	1.70	1020
I.POX_ov	30^a			0.113	7.37	737^c
Primary Uptake (%)	86^d
Washed Enzyme (%)	14^d
Effective load (%)	73
Activity Recovery (%)	14^e

^a15 mg per mL, ^bbased on the extinction coefficient of III_d at 375 nm pH 5 (15,330 cm⁻¹ M⁻¹), ^ctotal amount of I.POX_ov = 300 mg, ^dbased on absorbance at 403 nm, ^ebased on total units of activity
Total Protein: 0.258 mg specific activity = 27315.5 (see the supplementary materials for the calculations)

ISSN: 2468-2039