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Table 4 Comparison of LMWC products obtained by enzymatic hydrolysis method

From: Preparation, characterization and application in environmental protection of low-molecular-weight chitosan: a review

Enzyme

pH

T (°C)

Time (h)

Yield (%)

M W (kDa)

Ref.

pepsin

5.0

38

4

 

3.0

[60]

pepsin + papain

4.8

50

24

13

0.6

[61]

chitinase + cellulase

5.3

45

6

80

1.3

[62]

muramidase

4.0

42

72

 

22.2

[63]

amylase

5.5

55

4

96

 

[64]

pectinase

3.0

38

6

86

 

[65]

cellulase

5.2

55

24

 

2.2

[66]

neutral protease

5.4

50

4

94

25.7

[67]

cellulase

5.2

55

18

 

3.3

[68]

chitosanase

5.6

55

1.5

 

1–100

[69]