Fig. 8

The degree of fibrosis was observed by picrosirius red staining (a–f) and the Masson’s Trichrome stain (h–m). Mice were fed the ND (a, c, e, h, j and l) or HFD (b, d, f, i, k and m) for 25 weeks and treated with 0 (a, b, h and i), 100 (c, d, j and k), or 300 (e, f, l and m) mg of SNPs kg⁻¹ body weight twice per week. The arrow indicates positive picrosirius red staining. #, p < 0.001 for the comparison of the HFD-SNP0 group with the ND-SNP0 group, as revealed by ANOVA. The scale bars represent 100 µm (a–f, h–m). The degree of fibrosis was quantified using Image-Pro (g, n). The data are expressed as the means ± standard errors of the means (n = 6)